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frontiers / frontier

Perturbation-response biology

CC-BY-4.0vfr_4c9cf99e3883e2a2

id: vfr_4c9cf99e3883e2a2
license: CC-BY-4.0
findings: 200
accepted core: 0
contested: 0

200

findings

links

200

sources

200

evidence

contested

0.59

avg conf

frontiers / frontier

Perturbation-response biology

CC-BY-4.0vfr_4c9cf99e3883e2a2

id: vfr_4c9cf99e3883e2a2
license: CC-BY-4.0
findings: 200
accepted core: 0
contested: 0

200

findings

links

200

sources

200

evidence

contested

0.59

avg conf

Finding bundle

back to state

CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons

no incoming links yet

id: vf_64bbfd60d14def14
frontier: Perturbation-response biology
version: 1
confidence: 0.78

record state

frontier-owned

Review status

This finding is part of accepted frontier state. Review events, reviewable changes, and proof state explain how it can change.

unreviewed

finding statement

finding type

methodological

No entity list is declared.

evidence

source-bound

1 atoms

experimental · manual state transition

proof impact

packet context

1 events

1 reviewable changes and 0 evaluation records are attached to this finding id.

Evidence and conditions

method

manual state transition

evidence type

experimental

conditions

human_data: true
in_vitro: true
species_unverified
species_verified: Homo sapiens
text: CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based screens were conducted in cancer cell lines rather than healthy, differentiated cells. Here, we describe a CRISPR interference (CRISPRi)-based platform for genetic screens in human neurons derived from induced pluripotent stem cells (iPSCs). We demonstrate robust and durable knockdown of endogenous genes in such neurons and present result

Provenance

source title

Tian R;Gachechiladze MA;Ludwig CH;Laurie MT;Hong JY;Nathaniel D. Neuron 2019. doi:10.1016/j.neuron.2019.07.014

doi

10.1016/j.neuron.2019.07.014

journal

Neuron

authors

Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D

Source records

source record

declared

Tian R;Gachechiladze MA;Ludwig CH;Laurie MT;Hong JY;Nathaniel D. Neuron 2019. doi:10.1016/j.neuron.2019.07.014

vs_289f3db99117305e

doi:10.1016/j.neuron.2019.07.014 · 10.1016/j.neuron.2019.07.014

2019manual_curation

inspect source →

Evidence atoms

vea_7868856ca9d32d2eexperimental · unknown
CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons
vs_289f3db99117305e · manual_curation

Typed links

outgoing

No outgoing links.

incoming

No incoming links.

Review, event, and evaluation records

events

vev_09ed058acccf645bfinding.asserted
Manual finding added to frontier state
reviewer:will-blair-bot · 2026-05-05

reviewable changes

vpr_444a75264925e331finding.add
Manual finding added to frontier state
applied · reviewer:will-blair-bot · 2026-05-05

evaluations

No evaluation record targets this finding id.

Finding bundle

back to state

CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons

no incoming links yet

id: vf_64bbfd60d14def14
frontier: Perturbation-response biology
version: 1
confidence: 0.78

record state

frontier-owned

Review status

This finding is part of accepted frontier state. Review events, reviewable changes, and proof state explain how it can change.

unreviewed

finding statement

finding type

methodological

No entity list is declared.

evidence

source-bound

1 atoms

experimental · manual state transition

proof impact

packet context

1 events

1 reviewable changes and 0 evaluation records are attached to this finding id.

Evidence and conditions

method

manual state transition

evidence type

experimental

conditions

human_data: true
in_vitro: true
species_unverified
species_verified: Homo sapiens
text: CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based screens were conducted in cancer cell lines rather than healthy, differentiated cells. Here, we describe a CRISPR interference (CRISPRi)-based platform for genetic screens in human neurons derived from induced pluripotent stem cells (iPSCs). We demonstrate robust and durable knockdown of endogenous genes in such neurons and present result

Provenance

source title

Tian R;Gachechiladze MA;Ludwig CH;Laurie MT;Hong JY;Nathaniel D. Neuron 2019. doi:10.1016/j.neuron.2019.07.014

doi

10.1016/j.neuron.2019.07.014

journal

Neuron

authors

Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D

Source records

source record

declared

Tian R;Gachechiladze MA;Ludwig CH;Laurie MT;Hong JY;Nathaniel D. Neuron 2019. doi:10.1016/j.neuron.2019.07.014

vs_289f3db99117305e

doi:10.1016/j.neuron.2019.07.014 · 10.1016/j.neuron.2019.07.014

2019manual_curation

inspect source →

Evidence atoms

vea_7868856ca9d32d2eexperimental · unknown
CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons
vs_289f3db99117305e · manual_curation

Typed links

outgoing

No outgoing links.

incoming

No incoming links.

Review, event, and evaluation records

events

vev_09ed058acccf645bfinding.asserted
Manual finding added to frontier state
reviewer:will-blair-bot · 2026-05-05

reviewable changes

vpr_444a75264925e331finding.add
Manual finding added to frontier state
applied · reviewer:will-blair-bot · 2026-05-05

evaluations

No evaluation record targets this finding id.

Search Canopus

Review status

methodological

1 atoms

1 events

Source records

Tian R;Gachechiladze MA;Ludwig CH;Laurie MT;Hong JY;Nathaniel D. Neuron 2019. doi:10.1016/j.neuron.2019.07.014

Evidence atoms

Typed links

Review, event, and evaluation records

Review status

methodological

1 atoms

1 events

Source records

Tian R;Gachechiladze MA;Ludwig CH;Laurie MT;Hong JY;Nathaniel D. Neuron 2019. doi:10.1016/j.neuron.2019.07.014

Evidence atoms

Typed links

Review, event, and evaluation records