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Perturbation-response biology

CC-BY-4.0vfr_4c9cf99e3883e2a2

id: vfr_4c9cf99e3883e2a2
license: CC-BY-4.0
findings: 200
accepted core: 0
contested: 0

200

findings

links

200

sources

200

evidence

contested

0.59

avg conf

frontiers / frontier

Perturbation-response biology

CC-BY-4.0vfr_4c9cf99e3883e2a2

id: vfr_4c9cf99e3883e2a2
license: CC-BY-4.0
findings: 200
accepted core: 0
contested: 0

200

findings

links

200

sources

200

evidence

contested

0.59

avg conf

Finding bundle

back to state

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing

no incoming links yet

id: vf_8346ce422532d325
frontier: Perturbation-response biology
version: 1
confidence: 0.78

record state

frontier-owned

Review status

This finding is part of accepted frontier state. Review events, reviewable changes, and proof state explain how it can change.

unreviewed

finding statement

finding type

mechanism

No entity list is declared.

evidence

source-bound

1 atoms

experimental · manual state transition

proof impact

packet context

1 events

1 reviewable changes and 0 evaluation records are attached to this finding id.

Evidence and conditions

method

manual state transition

evidence type

experimental

conditions

species_unverified
species_verified: Mus musculus
text: Single-cell CRISPR screens enable the exploration of mammalian gene function and genetic regulatory networks. However, use of this technology has been limited by reliance on indirect indexing of single-guide RNAs (sgRNAs). Here we present direct-capture Perturb-seq, a versatile screening approach in which expressed sgRNAs are sequenced alongside single-cell transcriptomes. Direct-capture Perturb-seq enables detection of multiple distinct sgRNA sequences from individual cells

Provenance

source title

Replogle JM;Norman TM;Xu A;Hussmann JA;Chen J;Cogan JZ. Nature biotechnology 2020. doi:10.1038/s41587-020-0470-y

doi

10.1038/s41587-020-0470-y

journal

Nature biotechnology

authors

Replogle JM, Norman TM, Xu A, Hussmann JA, Chen J, Cogan JZ

Source records

source record

declared

Replogle JM;Norman TM;Xu A;Hussmann JA;Chen J;Cogan JZ. Nature biotechnology 2020. doi:10.1038/s41587-020-0470-y

vs_e69811e18af9710a

doi:10.1038/s41587-020-0470-y · 10.1038/s41587-020-0470-y

2020manual_curation

inspect source →

Evidence atoms

vea_92b289e2de009acaexperimental · unknown
Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing
vs_e69811e18af9710a · manual_curation

Typed links

outgoing

No outgoing links.

incoming

No incoming links.

Review, event, and evaluation records

events

vev_4275c27aee35a7d7finding.asserted
Manual finding added to frontier state
reviewer:will-blair-bot · 2026-05-05

reviewable changes

vpr_6393eb0effe2f90ffinding.add
Manual finding added to frontier state
applied · reviewer:will-blair-bot · 2026-05-05

evaluations

No evaluation record targets this finding id.

Finding bundle

back to state

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing

no incoming links yet

id: vf_8346ce422532d325
frontier: Perturbation-response biology
version: 1
confidence: 0.78

record state

frontier-owned

Review status

This finding is part of accepted frontier state. Review events, reviewable changes, and proof state explain how it can change.

unreviewed

finding statement

finding type

mechanism

No entity list is declared.

evidence

source-bound

1 atoms

experimental · manual state transition

proof impact

packet context

1 events

1 reviewable changes and 0 evaluation records are attached to this finding id.

Evidence and conditions

method

manual state transition

evidence type

experimental

conditions

species_unverified
species_verified: Mus musculus
text: Single-cell CRISPR screens enable the exploration of mammalian gene function and genetic regulatory networks. However, use of this technology has been limited by reliance on indirect indexing of single-guide RNAs (sgRNAs). Here we present direct-capture Perturb-seq, a versatile screening approach in which expressed sgRNAs are sequenced alongside single-cell transcriptomes. Direct-capture Perturb-seq enables detection of multiple distinct sgRNA sequences from individual cells

Provenance

source title

Replogle JM;Norman TM;Xu A;Hussmann JA;Chen J;Cogan JZ. Nature biotechnology 2020. doi:10.1038/s41587-020-0470-y

doi

10.1038/s41587-020-0470-y

journal

Nature biotechnology

authors

Replogle JM, Norman TM, Xu A, Hussmann JA, Chen J, Cogan JZ

Source records

source record

declared

Replogle JM;Norman TM;Xu A;Hussmann JA;Chen J;Cogan JZ. Nature biotechnology 2020. doi:10.1038/s41587-020-0470-y

vs_e69811e18af9710a

doi:10.1038/s41587-020-0470-y · 10.1038/s41587-020-0470-y

2020manual_curation

inspect source →

Evidence atoms

vea_92b289e2de009acaexperimental · unknown
Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing
vs_e69811e18af9710a · manual_curation

Typed links

outgoing

No outgoing links.

incoming

No incoming links.

Review, event, and evaluation records

events

vev_4275c27aee35a7d7finding.asserted
Manual finding added to frontier state
reviewer:will-blair-bot · 2026-05-05

reviewable changes

vpr_6393eb0effe2f90ffinding.add
Manual finding added to frontier state
applied · reviewer:will-blair-bot · 2026-05-05

evaluations

No evaluation record targets this finding id.

Search Canopus

Review status

mechanism

1 atoms

1 events

Source records

Replogle JM;Norman TM;Xu A;Hussmann JA;Chen J;Cogan JZ. Nature biotechnology 2020. doi:10.1038/s41587-020-0470-y

Evidence atoms

Typed links

Review, event, and evaluation records

Review status

mechanism

1 atoms

1 events

Source records

Replogle JM;Norman TM;Xu A;Hussmann JA;Chen J;Cogan JZ. Nature biotechnology 2020. doi:10.1038/s41587-020-0470-y

Evidence atoms

Typed links

Review, event, and evaluation records